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Antibodies sure to the blocking peptide now not bind for the epitope about the goal protein. By evaluating the staining within the blocked antibody compared to the antibody alone, one can see which staining is specific.
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This guidebook outlines further achievable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This manual outlines even further feasible optimization of cellular and immunoassay parameters to make sure the absolute best outcomes are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally more info antibodies...
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide commonly includes the epitope recognized via the antibody.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic event. Cyclin E varieties a fancy with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development as a result of S phase.
Overexpression of this gene is observed in lots of tumors, which leads to chromosome instability, and so may perhaps lead to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial purpose in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two supplemental splice variants were being reported but in-depth nucleotide sequence details is not really however readily available.
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